Suzuki Hideaki
   Department   School of Medicine  Otorhinolaryngology-Head and Neck Surgery, Clinical Medical Sciences
   Position  
Article types journal article
Language English
Peer review Peer reviewed
Title Possible contribution of pannexin-1 to capsaicin-induced ATP release in rat nasal columnar epithelial cells.
Journal Formal name:Channels (Austin, Tex.)
ISSN code:19336950
Domestic / ForeginForegin
Volume, Issue, Page 11(4),273-280頁
Author and coauthor Ohbuchi T, Do BH, Koizumi H, Takeuchi S, Ueta Y, Suzuki H
Publication date 2017/07
Summary Current evidence indicates that transient receptor potential (TRP) channel activity involves a relationship between opening of pannexin-1 and release of ATP into the extracellular space. We examined the effects of agonists of thermosensitive TRP channels (TRPM8, TRPA1, TRPV1, and TRPV2) on ATP release from rat nasal mucosa, and measured ciliary beat frequency (CBF) using digital high-speed video imaging. Single-cell patch clamping from dissociated rat nasal columnar epithelial cells was performed to confirm the relationship between pannexin-1 and TRP. We demonstrated that ATP release and CBF were significantly potentiated by the heat-sensitive TRPV1 agonist capsaicin (10 μM), but not by other TRP agonists. Capsaicin-induced ATP release and CBF increase were significantly inhibited by the pannexin-1 blockers carbenoxolone (10 μM) and probenecid (300 μM). In addition, the voltage step-evoked currents in the presence of capsaicin were inhibited by the pannexin-1 blockers in single-cell patch clamping. Our results suggest the participation of TRPV1 and pannexin-1 in the physiologic functions of rat nasal mucosa.
DOI 10.1080/19336950.2017.1293209
PMID 28631948