Suzuki Hideaki
   Department   School of Medicine  Otorhinolaryngology-Head and Neck Surgery, Clinical Medical Sciences
Article types journal article
Language English
Peer review Peer reviewed
Title Acetylcholine-induced ex vivo ATP release from the human nasal mucosa.
Journal Formal name:Auris, nasus, larynx
Abbreviation:Auris Nasus Larynx
ISSN code:18791476/03858146
Domestic / ForeginForegin
Volume, Issue, Page 44(4),422-427頁
Author and coauthor Koizumi Hiroki, Ikezaki Shoji, Ohbuchi Toyoaki, Do Ba Hung, Hohchi Nobusuke, Kawaguchi Rintaro, Kitamura Takuro, Suzuki Hideaki
Publication date 2017/08
Summary OBJECTIVE:The present study aimed at investigating ATP release in response to acetylcholine (Ach) and pharmacologically elucidating the intracellular signal transduction pathway of this reaction in an ex vivo experiment.METHODS:The inferior turbinate mucosa was collected from 21 patients with chronic hypertrophic rhinitis who underwent endoscopic turbinectomy. The mucosa was shaped into a filmy round piece, and incubated with chemical(s) in Hank's balanced salt solution for 10min. After incubation, the ATP concentration was measured by a luciferin-luciferase assay.RESULTS:The baseline release of ATP without stimulus was 57.2±10.3fM. The ATP release was significantly increased by stimulation with 100μM Ach. The Ach-induced ATP release was completely inhibited by removing extracellular Ca(2+). Significant inhibition of the Ach-induced ATP release was also observed by the addition of 1μM atropine, 40μM 2-APB, 10μM CBX, and 100μM PPADS, whereas 30nM bafilomycin A1 did not affect the ATP release.CONCLUSION:These results indicate that the Ach-induced ATP release from the human nasal mucosa is dependent on the pannexin-1 channel and purinergic P2X7 receptor, suggesting that these two molecules constitute a local autocrine/paracrine signaling system in the human nasal epithelium.
DOI 10.1016/j.anl.2016.09.003
PMID 27692399