Fujimoto Naohiro
   Department   School of Medicine  Urology, Clinical Medical Sciences
Article types journal article
Language English
Peer review Peer reviewed
Title Localization of type-2 angiotensin II receptor in adrenal gland.
Journal Formal name:The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Abbreviation:J Histochem Cytochem
ISSN code:15515044/00221554
Volume, Issue, Page 58(7),585-93頁
Author and coauthor Harada Keita, Matsuoka Hidetada, Fujimoto Naohiro, Endo Yutaka, Hasegawa Yoshitaka, Matsuo Akira, Kikuchi Yuta, Matsumoto Tetsuro, Inoue Masumi
Publication date 2010/07
Summary The localization of the type-2 angiotensin II receptor (AT2) in the adrenal glands of rats, guinea pigs, bovines, and humans was examined at the mRNA and protein levels. PCR products for AT2 were detected in the adrenal cortices and adrenal medullae of all the mammals examined with an RT-PCR technique. Three different anti-AT2 antibodies (Abs), whose specificity was confirmed in our hands, recognized a 50-kDa protein in the adrenal glands of the four mammals, and this recognition was abolished by the preabsorption of an Ab with an antigen. Immunoblotting and immunohistochemistry revealed that the 50-kDa protein was expressed consistently and variably in the adrenal cortices and medullae of various mammals, respectively. We conclude that the 50-kDa AT2 is consistently expressed in the adrenal cortex in a wide variety of mammals.
DOI 10.1369/jhc.2010.955575
PMID 20231739