Azuma Kagaku
   Department   School of Medicine  Anatomy(1), Biomedical Sciences
   Position  
Article types journal article
Language English
Peer review Peer reviewed
Title Expanded polyglutamine embedded in the endoplasmic reticulum causes membrane distortion and coincides with Bax insertion.
Journal Formal name:Biochemical and biophysical research communications
Abbreviation:Biochem Biophys Res Commun
ISSN code:10902104/0006291X
Domestic / ForeginForegin
Volume, Issue, Page 474(2),259-263頁
Author and coauthor Ueda Masashi, Li Shimo, Itoh Masanori, Wang Miao-Xing, Hayakawa Miki, Islam Saiful, Tana, Nakagawa Kiyomi, Chen Huayue, Nakagawa Toshiyuki
Publication date 2016/05
Summary The endoplasmic reticulum (ER) is important in various cellular functions, such as secretary and membrane protein biosynthesis, lipid synthesis, and calcium storage. ER stress, including membrane distortion, is associated with many diseases such as Huntington's disease. In particular, nuclear envelope distortion is related to neuronal cell death associated with polyglutamine. However, the mechanism by which polyglutamine causes ER membrane distortion remains unclear. We used electron microscopy, fluorescence protease protection assay, and alkaline treatment to analyze the localization of polyglutamine in cells. We characterized polyglutamine embedded in the ER membrane and noted an effect on morphology, including the dilation of ER luminal space and elongation of ER-mitochondria contact sites, in addition to the distortion of the nuclear envelope. The polyglutamine embedded in the ER membrane was observed at the same time as Bax insertion. These results demonstrated that the ER membrane may be a target of polyglutamine, which triggers cell death through Bax.
DOI 10.1016/j.bbrc.2016.04.034
PMID 27079237