アサカワ タケシ   Asakawa Takeshi
  浅川 剛
   所属   関連施設  情報管理センター 情報管理センター
   職種   准教授
論文種別 原著
言語種別
査読の有無 査読なし
表題 CLOCKΔ19 mutation modifies the manner of synchrony among oscillation neurons in the suprachiasmatic nucleus.
掲載誌名 正式名:Scientific reports
略  称:Sci Rep
ISSNコード:20452322/20452322
巻・号・頁 8(1),854頁
著者・共著者 Sujino Mitsugu, Asakawa Takeshi, Nagano Mamoru, Koinuma Satoshi, Masumoto Koh-Hei, Shigeyoshi Yasufumi
発行年月 2018/01
概要 In mammals, the principal circadian oscillator exists in the hypothalamic suprachiasmatic nucleus (SCN). In the SCN, CLOCK works as an essential component of molecular circadian oscillation, and ClockΔ19 mutant mice show unique characteristics of circadian rhythms such as extended free running periods, amplitude attenuation, and high-magnitude phase-resetting responses. Here we investigated what modifications occur in the spatiotemporal organization of clock gene expression in the SCN of ClockΔ19 mutants. The cultured SCN, sampled from neonatal homozygous ClockΔ19 mice on an ICR strain comprising PERIOD2::LUCIFERASE, demonstrated that the Clock gene mutation not only extends the circadian period, but also affects the spatial phase and period distribution of circadian oscillations in the SCN. In addition, disruption of the synchronization among neurons markedly attenuated the amplitude of the circadian rhythm of individual oscillating neurons in the mutant SCN. Further, with numerical simulations based on the present studies, the findings suggested that, in the SCN of the ClockΔ19 mutant mice, stable oscillation was preserved by the interaction among oscillating neurons, and that the orderly phase and period distribution that makes a phase wave are dependent on the functionality of CLOCK.
DOI 10.1038/s41598-018-19224-1
PMID 29339832