Zenke Yukichi
   Department   School of Medicine  Orthopedic Surgery, Clinical Medical Sciences
Article types journal article
Language English
Peer review Peer reviewed
Title Spontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold.
Journal Formal name:PloS one
Abbreviation:PLoS One
ISSN code:19326203/19326203
Domestic / ForeginForegin
Volume, Issue, Page 11(4),e0153231頁
Author and coauthor Sonomoto Koshiro, Yamaoka Kunihiro, Kaneko Hiroaki, Yamagata Kaoru, Sakata Kei, Zhang Xiangmei, Kondo Masahiro, Zenke Yukichi, Sabanai Ken, Nakayamada Shingo, Sakai Akinori, Tanaka Yoshiya
Publication date 2016
Summary INTRODUCTION:Mesenchymal stem cells (MSCs) have immunosuppressive activity and can differentiate into bone and cartilage; and thus seem ideal for treatment of rheumatoid arthritis (RA). Here, we investigated the osteogenesis and chondrogenesis potentials of MSCs seeded onto nano-fiber scaffolds (NFs) in vitro and possible use for the repair of RA-affected joints.METHODS:MSCs derived from healthy donors and patients with RA or osteoarthritis (OA) were seeded on poly-lactic-glycolic acid (PLGA) electrospun NFs and cultured in vitro.RESULTS:Healthy donor-derived MSCs seeded onto NFs stained positive with von Kossa at Day 14 post-stimulation for osteoblast differentiation. Similarly, MSCs stained positive with Safranin O at Day 14 post-stimulation for chondrocyte differentiation. Surprisingly, even cultured without any stimulation, MSCs expressed RUNX2 and SOX9 (master regulators of bone and cartilage differentiation) at Day 7. Moreover, MSCs stained positive for osteocalcin, a bone marker, and simultaneously also with Safranin O at Day 14. On Day 28, the cell morphology changed from a spindle-like to an osteocyte-like appearance with processes, along with the expression of dentin matrix protein-1 (DMP-1) and matrix extracellular phosphoglycoprotein (MEPE), suggesting possible differentiation of MSCs into osteocytes. Calcification was observed on Day 56. Expression of osteoblast and chondrocyte differentiation markers was also noted in MSCs derived from RA or OA patients seeded on NFs. Lactic acid present in NFs potentially induced MSC differentiation into osteoblasts.CONCLUSIONS:Our PLGA scaffold NFs induced MSC differentiation into bone and cartilage. NFs induction process resembled the procedure of endochondral ossification. This finding indicates that the combination of MSCs and NFs is a promising therapeutic technique for the repair of RA or OA joints affected by bone and cartilage destruction.
DOI 10.1371/journal.pone.0153231
PMID 27055270