ツジ マユミ   Tsuji Mayumi
  辻 真弓
   所属   医学部医学科  基礎医学系 衛生学
   職種   教授
論文種別 不明
言語種別
査読の有無 査読あり
表題 Inorganic mercury-induced MIP-2 expression is suppressed by N-acetyl-L-cysteine in RAW264.7 macrophages.
掲載誌名 正式名:Biomedical reports
略  称:Biomed Rep
ISSNコード:20499434/20499434
巻・号・頁 12(2),39-45頁
著者・共著者 David Juliet, Muniroh Muflihatul, Nandakumar Athira, Tsuji Mayumi, Koriyama Chihaya, Yamamoto Megumi
発行年月 2020/02
概要 Macrophages play an important role in neurotoxicity caused by methylmercury exposure through inflammatory responses. Methylmercury is known to demethylate to inorganic mercury in the brain, and macrophages are likely to be involved in this process. However, the inflammatory responses of macrophages against exposure to inorganic mercury are unclear. In the present study, inflammatory cytokine expression profiles were examined in the presence of non-toxic doses of inorganic mercury (Hg2+) using RAW264.7 macrophages, focusing on the expression of C-X-C motif chemokine 2 (MIP-2)/platelet-derived growth factor-inducible protein KC (KC) and C-C motif chemokine 12 (MCP-5). Furthermore, the suppressive effect of N-acetyl-L-cysteine (NAC) on inorganic mercury-induced MIP-2 expression was also examined. Inorganic mercury-induced mRNA expression was measured using reverse transcription-quantitative PCR. The mRNA expression of MIP-2 and MCP-5 was significantly upregulated by exposure to 20 µM Hg2+ (non-toxic levels), but not that of KC. The suppressive effect of NAC on these cytokine expression levels was examined by its addition to the culture medium together with Hg2+ (co-treatment), and pre- and post-treatments in which the cells were treated with NAC before and after Hg2+ exposure, respectively. Hg2+-upregulated MIP-2 expression was suppressed by NAC regardless of the time sequence of the treatment, suggesting that the suppressive role of NAC in Hg2+-induced inflammation manifests as a possible chelator and antioxidant/reactive oxygen species scavenger.
DOI 10.3892/br.2019.1263
PMID 31929872