ツジ マユミ   Tsuji Mayumi
  辻 真弓
   所属   医学部医学科  基礎医学系 衛生学
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Genome-wide evidences of bisphenol a toxicity using Schizosaccharomyces pombe.
掲載誌名 正式名:Archives of pharmacal research
略  称:Arch Pharm Res
ISSNコード:02536269/02536269
掲載区分国外
巻・号・頁 41(8),830-837頁
著者・共著者 Kim Dong-Myung, Heo Jeonghoon, Lee Dong Woo, Tsuji Mayumi, Yang Mihi
発行年月 2018/08
概要 To clarify reliable toxic mechanisms of bisphenol A (BPA), an endocrine disrupting chemical, we approached an alternative animal and whole genome analyses with the yeast knockout library (YKO) of Schizosaccharomyces pombe. As results, the 50% growth inhibition concentrations (GI50) of BPA was approximately 600 μM and the YKO-three step screening revealed the top 10 target candidate genes including dbp2, utp18, srs1, tif224, use1, qcr1, etc. The screening results were confirmed in human embryonic stem cell (hES)-derived hepatic cells and HepG2 human liver cancer cells. We found BPA down-regulated UQCRC, the human orthlog of S. pombe- qcr1, as a part of the mitochondrial respiratory chain, in HepG2 cells and hESs during cell differentiation into hepatic cells. Therefore, BPA may induce mitochondrial dysfunction and disruption of differentiation by suppressing UQCRC1.
DOI 10.1007/s12272-018-1058-7
PMID 30099677