ツジ マユミ   Tsuji Mayumi
  辻 真弓
   所属   医学部医学科  基礎医学系 衛生学
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Progression of vasogenic edema induced by activated microglia under permanent middle cerebral artery occlusion.
掲載誌名 正式名:Biochemical and biophysical research communications
略  称:Biochem Biophys Res Commun
ISSNコード:10902104/0006291X
巻・号・頁 496(2),582-587頁
著者・共著者 Tanaka Miki, Ishihara Yasuhiro, Mizuno Shodo, Ishida Atsuhiko, Vogel Christoph F, Tsuji Mayumi, Yamazaki Takeshi, Itoh Kouichi
発行年月 2018/02
概要 Brain edema is a severe complication that accompanies ischemic stroke. Increasing evidence shows that inflammatory cytokines impair tight junctions of the blood-brain barrier, suggesting the involvement of microglia in brain edema. In this study, we examined the role of microglia in the progression of ischemic brain edema using mice with permanent middle cerebral artery occlusion. The intensity of T2-weighted imaging (T2WI) in the cerebral cortex and the striatum was elevated 3 h after occlusion and spread to peripheral regions of the ischemic hemisphere. Merged images of 2,3,5-triphenyl tetrazolium chloride staining and T2WI revealed the exact vasogenic edema region, which spread from the ischemic core to outside the ischemic region. Microglia were strongly activated in the ischemic region 3 h after occlusion and, notably, activated microglia were observed in the non-ischemic region 24 h after occlusion. Pretreatment with minocycline, an inhibitor of microglial activation clearly suppressed not only vasogenic edema but also infarct formation. We demonstrated in this study that vasogenic edema spreads from the ischemic core to the peripheral region, which can be elicited, at least in part, by microglial activation induced by ischemia.
DOI 10.1016/j.bbrc.2018.01.094
PMID 29353043