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Shibata Eiji
Department School of Medicine Obstetrics and Gynecology, Clinical Medical Sciences Position |
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| Article types | journal article |
| Language | English |
| Peer review | Non peer reviewed |
| Title | Differential levels of amino acid transporters System L and ASCT2, and the mTOR protein in placenta of preeclampsia and IUGR. |
| Journal | Formal name：BMC pregnancy and childbirth Abbreviation：BMC Pregnancy Childbirth ISSN code：14712393/14712393 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 14(1),181頁 |
| Author and coauthor | Aiko Yukiyo, Askew David J, Aramaki Satoshi, Myoga Mai, Tomonaga Chiharu, Hachisuga Toru, Suga Reiko, Kawamoto Toshihiro, Tsuji Mayumi, Shibata Eiji |
| Publication date | 2014/05 |
| Summary | BACKGROUND:Sufficient amino acid transport activity (AAT) is indispensable for appropriate fetal growth. Studies suggest that placental nutrient uptake activity is responsive to both maternal and fetal nutrient demands. We hypothesize that under conditions of limited nutrient availability to the fetus, as often present in preeclampsia, intrauterine growth restriction (IUGR), and insufficient weight-gain during pregnancy, a general adaptive response aimed to increase amino acid transport activity may be observed in the placenta.METHOD:A total of 40 placentas from full-term (n = 10) and pre-term (average gestational period = 34.8 weeks, n = 10) normal pregnancies, IUGR (n = 10), and preeclampsia (n = 10) associated pregnancies were looked at by immunohistochemistry followed by relative qualitative scoring to compare expression levels and localization of System L, ASCT2, and mTOR proteins.RESULT:Microvillous syncytiotrophoblast (ST) in placenta of pregnancies complicated by IUGR or preeclampsia (PE) showed significant increases in the levels of System L amino acid transport proteins 4F2hc and LAT1 compared to both full-term control and pre-term (early gestation control) pregnancies seperately (p < 0.05). Elevated mTOR protein was uniquely higher in IUGR placentas compared to full-term controls (P = 0.0026). Total cellular ASCT2 transporter protein levels were similar in all groups, however, levels of ASCT2 protein localized to the ST microvillous membrane (MVM) were significantly lower in IUGR compared to both full-term and pre-term pregnancies (P = 0.0006, 0.03, respectively). Additionally, ASCT2 and mTOR protein levels were positively associated with maternal pre-pregnancy BMI (P = 0.046, 0.048, respectively).CONCLUSION:There are three important findings based upon the present study. First, in conditions of limited nutrient availability, such as PE or IUGR, there is an overall increase in the level of System L and mTOR protein expr |
| DOI | 10.1186/1471-2393-14-181 |
| PMID | 24886642 |