Nakano Yoshiteru
   Department   School of Medicine  Neurosurgery, Clinical Medical Sciences
Article types journal article
Language English
Peer review Peer reviewed
Title 1-BP inhibits NF-kappaB activity and Bcl-xL expression in astrocytes in vitro and reduces Bcl-xL expression in the brains of rats in vivo.
Journal Formal name:Neurotoxicology
ISSN code:0161813X/0161813X
Volume, Issue, Page 28(2),381-6頁
Author and coauthor Yoshida Y, Liu J Q, Nakano Y, Ueno S, Ohmori S, Fueta Y, Ishidao T, Kunugita N, Yamashita U, Hori H
Publication date 2007/03
Summary 1-Bromopropane (1-BP) has been widely used as a substitute for chlorofluorocarbon that destroys the ozone layer. Although the central neurotoxicity of 1-BP has been recently reported, a molecular mechanism is not clear. In particular, the effects on cells in brain have not been fully analyzed. Here, we studied the effects of 1-BP on the activation of transcription factors involved in anti-apoptotic function or cell survival in astrocytes. Astrocytoma cell lines, U251, U373 and VM, or murine primary astrocytes were used for in vitro assay. DNA binding activities of NF-kappaB in these cells induced by interleukin (IL)-1 or LPS were inhibited by 1-BP. Consequently, the treatment of U251 cells with 1-BP resulted in suppression of NF-kappaB reporter activity. Furthermore, 1-BP blocked IkappaBalpha degradation, which is important for NF-kappaB activation. In addition, the level of Bcl-xL mRNA, which is known as an anti-apoptotic gene, were reduced in U251 treated with 1-BP or in the brain from rat exposed to 1-BP (400 ppm, 12 weeks). These results suggest that subchronic inhalation exposure to 1-BP vapor may affect the Bcl-xL expression in astrocytes.
DOI 10.1016/j.neuro.2006.05.015
PMID 16815550