Mori Masataka
   Department   University Hospital  General Thoracic Surgery, Clinical Departments
Article types journal article
Language English
Peer review Peer reviewed
Title Detection of circulating tumor cells with a novel microfluidic system in malignant pleural mesothelioma
Journal Formal name:Cancer science
Abbreviation:Cancer Sci
ISSN code:13479032
Domestic / ForeginForegin
Volume, Issue, Page 110(2),726-733頁
Author and coauthor Kazue Yoneda, 1 Taiji Kuwata, 1 Yasuhiro Chikaishi, 1 Masataka Mori, 1 Masatoshi Kanayama, 1 Masaru Takenaka, 1 Soichi Oka, 1 Ayako Hirai, 1 Naoko Imanishi, 1 Koji Kuroda, 1 Yoshinobu Ichiki, 1 Takashi Ohnaga, 2 and Fumihiro Tanaka 1
Publication date 2019/02
Summary Detection of rare tumor cells circulating in the blood (CTCs) presents technical challenges. CellSearch, the only approved system for clinical use, fails to capture epithelial cell adhesion molecule-negative CTCs such as malignant pleural mesothelioma (MPM). We have developed a novel microfluidic device (CTC-chip) in which any Ab to capture CTCs is conjugated. The CTC-chip was coated with an Ab against podoplanin that is abundantly expressed on MPM. Circulating tumor cell-detection performance was evaluated in experimental models in which MPM cells were spiked in blood sampled from a healthy volunteer and in clinical samples drawn from MPM patients. The CTC-chip showed superior CTC-detection performance over CellSearch in experimental models (sensitivity, 63.3%-64.5% vs 0%-1.1%; P < .001) and in clinical samples (CTC-positivity, 68.8% vs 6.3%; P < .001). A receiver operating characteristic (ROC) analysis showed that the CTC test provided a significant diagnostic performance in discrimination of unresectable disease from resectable disease (area under the ROC curve, 0.851; P = .003). The higher CTC count (≥2 cells/mL) was significantly associated with a poor prognosis (P = .030). The novel CTC-chip enabled sensitive detection of CTCs, which provided significant diagnostic and prognostic information in MPM.
DOI 10.1111/cas.13895
PMID 30499156