Aramaki Satoshi
   Department   University Hospital  General Perinatal Medical Center, University Hospital
Article types journal article
Language English
Peer review Non peer reviewed
Title Differential levels of amino acid transporters System L and ASCT2, and the mTOR protein in placenta of preeclampsia and IUGR.
Journal Formal name:BMC pregnancy and childbirth
Abbreviation:BMC Pregnancy Childbirth
ISSN code:14712393/14712393
Domestic / ForeginForegin
Volume, Issue, Page 14(1),181頁
Author and coauthor Aiko Yukiyo, Askew David J, Aramaki Satoshi, Myoga Mai, Tomonaga Chiharu, Hachisuga Toru, Suga Reiko, Kawamoto Toshihiro, Tsuji Mayumi, Shibata Eiji
Publication date 2014/05
Summary BACKGROUND:Sufficient amino acid transport activity (AAT) is indispensable for appropriate fetal growth. Studies suggest that placental nutrient uptake activity is responsive to both maternal and fetal nutrient demands. We hypothesize that under conditions of limited nutrient availability to the fetus, as often present in preeclampsia, intrauterine growth restriction (IUGR), and insufficient weight-gain during pregnancy, a general adaptive response aimed to increase amino acid transport activity may be observed in the placenta.METHOD:A total of 40 placentas from full-term (n = 10) and pre-term (average gestational period = 34.8 weeks, n = 10) normal pregnancies, IUGR (n = 10), and preeclampsia (n = 10) associated pregnancies were looked at by immunohistochemistry followed by relative qualitative scoring to compare expression levels and localization of System L, ASCT2, and mTOR proteins.RESULT:Microvillous syncytiotrophoblast (ST) in placenta of pregnancies complicated by IUGR or preeclampsia (PE) showed significant increases in the levels of System L amino acid transport proteins 4F2hc and LAT1 compared to both full-term control and pre-term (early gestation control) pregnancies seperately (p < 0.05). Elevated mTOR protein was uniquely higher in IUGR placentas compared to full-term controls (P = 0.0026). Total cellular ASCT2 transporter protein levels were similar in all groups, however, levels of ASCT2 protein localized to the ST microvillous membrane (MVM) were significantly lower in IUGR compared to both full-term and pre-term pregnancies (P = 0.0006, 0.03, respectively). Additionally, ASCT2 and mTOR protein levels were positively associated with maternal pre-pregnancy BMI (P = 0.046, 0.048, respectively).CONCLUSION:There are three important findings based upon the present study. First, in conditions of limited nutrient availability, such as PE or IUGR, there is an overall increase in the level of System L and mTOR protein expr
DOI 10.1186/1471-2393-14-181
PMID 24886642